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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated DACH1 Polyclonal antibody

DACH1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10914

Print datasheet

Synonyms

Dachshund-1, DACH

验证数据展示

  • IF Staining of HEK-293 using CL594-10914

    IF Staining of HEK-293 using CL594-10914

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 DACH1 antibody (CL594-10914) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy DACH1 Ready-To-Use IHC Kit. DACH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10914-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10914 targets DACH1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen DACH1 fusion protein Ag1354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dachshund homolog 1 (Drosophila)
Synonyms Dachshund-1, DACH
Calculated Molecular Weight 79 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
RRIDAB_2919754
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI36
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human DACH1.

实验方案

Product Specific Protocols
IF protocol for CL594 DACH1 antibody CL594-10914Download protocol
Standard Protocols
Click here to view our Standard Protocols