验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
For other applications, we recommend the unconjugated version of this antibody, 22524-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-22524 targets DAT in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | DAT fusion protein Ag18297 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
| 别名 | DA transporter, DAT, DAT1, SLC6A3 |
| 计算分子量 | 620 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC133003 |
| 基因名称 | DAT |
| Gene ID (NCBI) | 6531 |
| RRID | AB_3672743 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01959 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DAT antibody CL488-22524 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |