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DAXX Monoclonal antibody

DAXX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G9

Cat no : 67879-1-Ig

Print datasheet

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

验证数据展示

  • WB analysis of Raji using 67879-1-Ig

    WB analysis of Raji using 67879-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67879-1-Ig

    IHC staining of human breast cancer using 67879-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DAXX antibody included
  • IF Staining of A431 using 67879-1-Ig

    IF Staining of A431 using 67879-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inRaji cells, HeLa cells, Daudi cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67879-1-Ig targets DAXX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen DAXX fusion protein Ag14606 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name death-domain associated protein
Synonyms BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx
Calculated Molecular Weight 740 aa, 81 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
RRIDAB_2918636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UER7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

实验方案

Product Specific Protocols
WB protocol for DAXX antibody 67879-1-IgDownload protocol
IHC protocol for DAXX antibody 67879-1-IgDownload protocol
IF protocol for DAXX antibody 67879-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols