验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67879-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67879 targets DAXX in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | DAXX fusion protein Ag14606 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | death-domain associated protein |
| 别名 | BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx |
| 计算分子量 | 740 aa, 81 kDa |
| 观测分子量 | 100-130 kDa |
| GenBank蛋白编号 | BC109074 |
| 基因名称 | DAXX |
| Gene ID (NCBI) | 1616 |
| RRID | AB_2934581 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UER7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DAXX antibody CL488-67879 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |