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验证数据展示

  • IF Staining of A431 using CL488-67879

    IF Staining of A431 using CL488-67879

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DAXX antibody (CL488-67879, Clone: 1F3G9 ) at dilution of 1:400.

CoraLite® Plus 488-conjugated DAXX Monoclonal antibody

DAXX Monoclonal Antibody for IF/ICC
Cat No. CL488-67879

Print datasheet

CloneNo. 1F3G9

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DAXX Ready-To-Use IHC Kit. DAXX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67879-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67879 targets DAXX in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen DAXX fusion protein Ag14606 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name death-domain associated protein
Synonyms BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx
Calculated Molecular Weight 740 aa, 81 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
RRIDAB_2934581
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UER7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DAXX antibody CL488-67879Download protocol
Standard Protocols
Click here to view our Standard Protocols