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验证数据展示

  • IF Staining of mouse brain using CL488-13925

    IF Staining of mouse brain using CL488-13925

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 DCX antibody (CL488-13925) at dilution of 1:50. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated DCX Polyclonal antibody

DCX Polyclonal Antibody for IF-P
Cat No. CL488-13925

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Synonyms

Lis-X, Lissencephalin-X, Doublin, doublecortin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue

This antibody is not recommended for IHC test.

For other applications, we recommend the unconjugated version of this antibody, 13925-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13925 targets DCX in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen DCX fusion protein Ag4945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name doublecortin
Synonyms Lis-X, Lissencephalin-X, Doublin, doublecortin
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
RRIDAB_3672579
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43602
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCX antibody CL488-13925Download protocol
Standard Protocols
Click here to view our Standard Protocols