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CHOP; GADD153 Polyclonal antibody
CHOP; GADD153 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
467
验证数据展示
经过测试的应用
Positive WB detected in | Tunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IP detected in | C6 cells |
Positive IHC detected in | human colon cancer tissue, mouse brain tissue, human breast cancer tissue, human thyroid cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Tunicamycin treated HeLa cells |
Positive FC detected in | HeLa cells |
Samples need to be treated with ER stress.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:500 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15204-1-AP targets CHOP; GADD153 in WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP, ChIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, zebrafish, Hamster, pig, Bovine |
Immunogen | CHOP; GADD153 fusion protein Ag7354 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DNA-damage-inducible transcript 3 |
Synonyms | C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153 |
Calculated Molecular Weight | 19 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC003637 |
Gene Symbol | DDIT3 |
Gene ID (NCBI) | 1649 |
RRID | AB_2292610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P35638 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
IHC protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
IF protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
IP protocol for CHOP; GADD153 antibody 15204-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis | ||
Mol Cell The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation |