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  • Featured Product
  • KD/KO Validated

CHOP/GADD153 Polyclonal antibody

CHOP/GADD153 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 15204-1-AP

Print datasheet

Synonyms

CHOP; GADD153, CHOP, DDIT3, GADD153, CCAAT/enhancer-binding protein homologous protein

验证数据展示

  • WB analysis of HeLa using 15204-1-AP

    WB analysis of HeLa using 15204-1-AP

    WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.

  • WB analysis using 15204-1-AP

    WB analysis using 15204-1-AP

    Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15204-1-AP

    IHC staining of human breast cancer using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IHC staining of human colon cancer using 15204-1-AP

    IHC staining of human colon cancer using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IHC staining of human colon cancer using 15204-1-AP

    IHC staining of human colon cancer using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IHC staining of human cervical cancer using 15204-1-AP

    IHC staining of human cervical cancer using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IF Staining of HeLa using 15204-1-AP

    IF Staining of HeLa using 15204-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CHOP; GADD153 antibody (15204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HeLa using 15204-1-AP

    FC experiment of HeLa using 15204-1-AP

    1X10^6 HeLa cells untreated (dashed lines) or treated with Tunicamycin (20 μg/mL, 4 h; red) were intracellularly stained with 0.4 ug Anti-Human CHOP; GADD153 (15204-1-AP) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IP experiment of C6 using 15204-1-AP

    IP experiment of C6 using 15204-1-AP

    IP result of anti-CHOP; GADD153 (IP:15204-1-AP, 4ug; Detection:15204-1-AP 1:4000) with C6 cells lysate 1600 ug.

  • IHC staining of mouse brain using 15204-1-AP

    IHC staining of mouse brain using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included
  • IHC staining of mouse brain using 15204-1-AP

    IHC staining of mouse brain using 15204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15204-1-AP (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDIT3 antibody included

经过测试的应用

Positive WB detected inTunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human thyroid cancer tissue, human cervical cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTunicamycin treated HeLa cells
Positive FC (Intra) detected inTunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15204-1-AP targets CHOP/GADD153 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, chicken, zebrafish, bovine, hamster
Immunogen CHOP/GADD153 fusion protein Ag7354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA-damage-inducible transcript 3
Synonyms CHOP; GADD153, CHOP, DDIT3, GADD153, CCAAT/enhancer-binding protein homologous protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
RRIDAB_2292610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35638
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

实验方案

Product Specific Protocols
WB protocol for CHOP/GADD153 antibody 15204-1-APDownload protocol
IHC protocol for CHOP/GADD153 antibody 15204-1-APDownload protocol
IF protocol for CHOP/GADD153 antibody 15204-1-APDownload protocol
IP protocol for CHOP/GADD153 antibody 15204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
View Article
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
View Article
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
View Article
humanWB,IHC,IP,ChIP

Mol Cell

The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.

Authors - Hayley J Zullow
View Article
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
View Article
humanWB

Cell Rep Med

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Authors - Yu Chang
View Article