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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHOP; GADD153 Polyclonal antibody

CHOP; GADD153 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15204

Print datasheet

Synonyms

CHOP, DDIT3, GADD153, CHOP10, CCAAT/enhancer-binding protein homologous protein

验证数据展示

  • IF Staining of HeLa using CL488-15204

    IF Staining of HeLa using CL488-15204

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CoraLite® Plus 488 CHOP; GADD153 antibody (CL488-15204) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inTunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15204-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15204 targets CHOP; GADD153 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen CHOP; GADD153 fusion protein Ag7354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DNA-damage-inducible transcript 3
Synonyms CHOP, DDIT3, GADD153, CHOP10, CCAAT/enhancer-binding protein homologous protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35638
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHOP; GADD153 antibody CL488-15204Download protocol
Standard Protocols
Click here to view our Standard Protocols