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验证数据展示

  • IF Staining of HeLa using CL488-15204

    IF Staining of HeLa using CL488-15204

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CoraLite® Plus 488 CHOP; GADD153 antibody (CL488-15204) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CHOP/GADD153 Polyclonal antibody

CHOP/GADD153 Polyclonal Antibody for IF/ICC
Cat No. CL488-15204

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

CHOP; GADD153, CHOP, DDIT3, GADD153, C/EBP zeta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15204-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15204 targets CHOP/GADD153 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 CHOP/GADD153 fusion protein Ag7354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA-damage-inducible transcript 3
别名 CHOP; GADD153, CHOP, DDIT3, GADD153, C/EBP zeta
计算分子量 19 kDa
观测分子量30 kDa
GenBank蛋白编号BC003637
基因名称 CHOP
Gene ID (NCBI) 1649
RRIDAB_3672624
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35638
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHOP/GADD153 antibody CL488-15204Download protocol
Standard Protocols
Click here to view our Standard Protocols