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DGCR8 Monoclonal antibody

DGCR8 Monoclonal Antibody for WB, IP, ELISA
Cat No. 60084-1-Ig

产品说明书

CloneNo. 4D11A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IP, ELISA and More (1)

C22orf12, DGCR8, DGCRK6, Gy1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, BxPC-3 cells, RAW 264.7 cells, A431 cells, Jurkat cells, K-562 cells, ROS1728 cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60084-1-Ig targets DGCR8 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IP, RIP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原 DGCR8 fusion protein Ag4871 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DiGeorge syndrome critical region gene 8
别名 C22orf12, DGCR8, DGCRK6, Gy1
计算分子量 773 aa, 86 kDa
观测分子量 120 kDa
GenBank蛋白编号BC009323
基因名称 DGCR8
Gene ID (NCBI) 54487
RRIDAB_2090985
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WYQ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

实验方案

Product Specific Protocols
WB protocol for DGCR8 antibody 60084-1-IgDownload protocol
IP protocol for DGCR8 antibody 60084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Nature

Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated
mouseWB

Mol Cell

A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation

Authors - Xuehui Lyu
humanWB

Nat Commun

Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.

Authors - Hailong Zhang
human,ratWB

Aging Cell

p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease

Authors - Haidong Xu
humanWB,RIP

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
human, mouseWB

Nucleic Acids Res

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Authors - Changhong Zhu
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