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验证数据展示

  • WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

    WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

    IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

    IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DGCR8 Monoclonal antibody, PBS Only

DGCR8 Monoclonal Antibody for WB, IP, Indirect ELISA
Cat No. 60084-1-PBS

产品说明书

CloneNo. 4D11A3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IP, Indirect ELISA

别名

C22orf12, DGCR8, DGCRK6, Gy1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60084-1-PBS targets DGCR8 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DGCR8 fusion protein Ag4871 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 DiGeorge syndrome critical region gene 8
别名 C22orf12, DGCR8, DGCRK6, Gy1
计算分子量 773 aa, 86 kDa
观测分子量 120 kDa
GenBank蛋白编号BC009323
基因名称 DGCR8
Gene ID (NCBI) 54487
RRIDAB_2090985
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WYQ5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.