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  • Featured Product
  • KD/KO Validated

DGCR8 Monoclonal antibody, PBS Only

DGCR8 Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D11A3

Cat no : 60084-1-PBS

Print datasheet

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

验证数据展示

  • WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

    WB analysis using 60084-1-Ig (same clone as 60084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

    IP experiment of K-562 using 60084-1-Ig (same clone as 60084-1-PBS)

    IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 60084-1-PBS in a different storage buffer formulation.

产品信息

60084-1-PBS targets DGCR8 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen DGCR8 fusion protein Ag4871 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DiGeorge syndrome critical region gene 8
Synonyms C22orf12, DGCR8, DGCRK6, Gy1
Calculated Molecular Weight 773 aa, 86 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009323
Gene Symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WYQ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.