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- KD/KO Validated
DLD Polyclonal antibody
DLD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16431-1-AP targets DLD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | DLD fusion protein Ag9729 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | dihydrolipoamide dehydrogenase |
| Synonyms | PHE3, LAD, GCSL, EC:1.8.1.4, DLDH |
| Calculated Molecular Weight | 509 aa, 54 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC018696 |
| Gene Symbol | DLD |
| Gene ID (NCBI) | 1738 |
| RRID | AB_2091888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09622 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DLD antibody 16431-1-AP | Download protocol |
| IHC protocol for DLD antibody 16431-1-AP | Download protocol |
| IF protocol for DLD antibody 16431-1-AP | Download protocol |
| IP protocol for DLD antibody 16431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis
| ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
| ||
Redox Biol Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways. |
