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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DLD Polyclonal antibody

DLD Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16431

Print datasheet

Synonyms

dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3

验证数据展示

  • IF Staining of HepG2 using CL488-16431

    IF Staining of HepG2 using CL488-16431

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 DLD antibody (CL488-16431) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DLD Ready-To-Use IHC Kit. DLD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16431-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16431 targets DLD in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen DLD fusion protein Ag9729 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dihydrolipoamide dehydrogenase
Synonyms dihydrolipoamide dehydrogenase, DLD, DLDH, E3, GCSL, LAD, PHE3
Calculated Molecular Weight 509 aa, 54 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC018696
Gene Symbol DLD
Gene ID (NCBI) 1738
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09622
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DLD antibody CL488-16431Download protocol
Standard Protocols
Click here to view our Standard Protocols