• Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DMT1 Polyclonal antibody

DMT1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20507

Print datasheet

Synonyms

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, FLJ37416, NRAMP 2, NRAMP2, SLC11A2



经过测试的应用

Positive IF-P detected inmouse small intestine tissue
For other applications, we recommend the unconjugated version of this antibody, 20507-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20507 targets DMT1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen DMT1 fusion protein Ag14314 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Synonyms DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT 1, DMT1, FLJ37416, NRAMP 2, NRAMP2, SLC11A2
Calculated Molecular Weight 568 aa, 62 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC002592
Gene Symbol DMT1
Gene ID (NCBI) 4891
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49281
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DMT1 antibody CL488-20507Download protocol
Standard Protocols
Click here to view our Standard Protocols