验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, mouse heart, mouse liver, rat liver |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human colon tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11305-1-AP targets ECHS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ECHS1 fusion protein Ag1842 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | enoyl Coenzyme A hydratase, short chain, 1, mitochondrial |
| 别名 | mECH1, mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM |
| 计算分子量 | 31 kDa |
| 观测分子量 | 29-31 kDa |
| GenBank蛋白编号 | BC008906 |
| 基因名称 | ECHS1 |
| Gene ID (NCBI) | 1892 |
| RRID | AB_2262166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30084 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| IHC protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| IF protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| IP protocol for ECHS1 antibody 11305-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Resist Updat MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance | ||
Cancer Res One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis. | ||
J Proteome Res Proteomic analysis of left ventricular remodeling in an experimental model of heart failure. | ||
J Proteome Res Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients. | ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
Am J Pathol The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach. |
