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验证数据展示

  • IF Staining of HeLa using CL488-11305

    IF Staining of HeLa using CL488-11305

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-11305) at dilution of 1:200.

  • IF Staining of HeLa using CL488-11305

    IF Staining of HeLa using CL488-11305

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-11305) at dilution of 1:1600.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for IF/ICC
Cat No. CL488-11305

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

ECHS1, Enoyl CoA hydratase 1, SCEH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11305-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11305 targets ECHS1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ECHS1 fusion protein Ag1842 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
别名 ECHS1, Enoyl CoA hydratase 1, SCEH
计算分子量 31 kDa
观测分子量 29-31 kDa
GenBank蛋白编号BC008906
基因名称 ECHS1
Gene ID (NCBI) 1892
RRIDAB_3083898
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30084
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ECHS1 antibody CL488-11305Download protocol
Standard Protocols
Click here to view our Standard Protocols