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EIF2AK2 Monoclonal antibody

EIF2AK2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8A4

Cat no : 66646-1-Ig

Print datasheet

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

验证数据展示

  • WB analysis using 66646-1-Ig

    WB analysis using 66646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 66646-1-Ig

    IHC staining of human testis using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2AK2 antibody included
  • IHC staining of human testis using 66646-1-Ig

    IHC staining of human testis using 66646-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2AK2 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF2AK2 Ready-To-Use IHC Kit. EIF2AK2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66646-1-Ig targets EIF2AK2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen EIF2AK2 fusion protein Ag13010 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2882004
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19525
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.

实验方案

Product Specific Protocols
WB protocol for EIF2AK2 antibody 66646-1-IgDownload protocol
IHC protocol for EIF2AK2 antibody 66646-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

The impact of single-stranded RNAs on the dimerization of double-stranded RNA-dependent protein kinase PKR

Authors - Tomoya Kitano
View Article