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EIF2AK2 Monoclonal antibody, PBS Only

EIF2AK2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8A4

Cat no : 66646-1-PBS

Print datasheet

Synonyms

eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated

验证数据展示

  • WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

    WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK2 antibody included
  • IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK2 antibody included
Planning an IHC experiment? We recommend our IHCeasy EIF2AK2 Ready-To-Use IHC Kit. EIF2AK2 primary antibody included.

产品信息

66646-1-PBS targets EIF2AK2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen EIF2AK2 fusion protein Ag13010 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms eIF 2A protein kinase 2, EIF2AK1, EIF2AK2, P1/eIF 2A protein kinase, p68 kinase, PKR, PRKR, Protein kinase RNA activated
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 65-68 kDa
GenBank Accession NumberBC093676
Gene Symbol EIF2AK2
Gene ID (NCBI) 5610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19525
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.