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  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11178-1-AP

Print datasheet

Synonyms

Rrp6p, RRP6, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component 10

验证数据展示

  • WB analysis of HeLa using 11178-1-AP

    WB analysis of HeLa using 11178-1-AP

    WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

  • IP experiment of MCF-7 using 11178-1-AP

    IP experiment of MCF-7 using 11178-1-AP

    IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

  • IHC staining of human breast cancer using 11178-1-AP

    IHC staining of human breast cancer using 11178-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with EXOSC10 antibody included
  • IF Staining of NIH/3T3 using 11178-1-AP

    IF Staining of NIH/3T3 using 11178-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11178-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen EXOSC10 fusion protein Ag1666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exosome component 10
Synonyms Rrp6p, RRP6, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, Exosome complex component 10
Calculated Molecular Weight 98 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2293792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

实验方案

Product Specific Protocols
WB protocol for EXOSC10 antibody 11178-1-APDownload protocol
IHC protocol for EXOSC10 antibody 11178-1-APDownload protocol
IF protocol for EXOSC10 antibody 11178-1-APDownload protocol
IP protocol for EXOSC10 antibody 11178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanWB

Cell Rep

Human Telomerase RNA Processing and Quality Control.

Authors - Chi-Kang Tseng
  • KD Validated
View Article
mouseWB,RIP

Development

Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.

Authors - Pauline Antonie Ulmke
  • KO Validated
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
View Article