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FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 11531-1-AP

Print datasheet

Synonyms

FBF-1, FBF 1, Fas-binding factor 1, Fas (TNFRSF6) binding factor 1, albatross



经过测试的应用

Positive IF/ICC detected inA549 cells, HUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11531-1-AP targets FBF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen FBF1 fusion protein Ag2106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fas (TNFRSF6) binding factor 1
Synonyms FBF-1, FBF 1, Fas-binding factor 1, Fas (TNFRSF6) binding factor 1, albatross
Calculated Molecular Weight1133 aa, 125 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC023549
Gene Symbol FBF1
Gene ID (NCBI) 85302
RRIDAB_2246683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TES7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

实验方案

Product Specific Protocols
IF protocol for FBF1 antibody 11531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
humanWB,IF

Nat Commun

Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner

Authors - Jielu Hao Robichaud
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanIF

Nat Commun

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Authors - Kai He
humanIF

Nat Commun

A distal centriolar protein network controls organelle maturation and asymmetry.

Authors - Lei Wang
humanIF,WB

Nat Commun

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Authors - Hao Yan