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验证数据展示

  • IF Staining of A549 using CL488-11531

    IF Staining of A549 using CL488-11531

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 FBF1 antibody (CL488-11531) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF/ICC
Cat No. CL488-11531

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells, HUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 11531-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11531 targets FBF1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 FBF1 fusion protein Ag2106 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fas (TNFRSF6) binding factor 1
别名 Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, FLJ00103, FLJ13996, HCG1989313, isoform CRA_b, KIAA1863
计算分子量1133 aa, 125 kDa
观测分子量 130 kDa
GenBank蛋白编号BC023549
基因名称 FBF1
Gene ID (NCBI) 85302
RRIDAB_2919031
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TES7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FBF1 antibody CL488-11531Download protocol
Standard Protocols
Click here to view our Standard Protocols