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验证数据展示

  • IF Staining of HeLa using CL488-11375

    IF Staining of HeLa using CL488-11375

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 FH antibody (CL488-11375) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FH Polyclonal antibody

FH Polyclonal Antibody for IF/ICC
Cat No. CL488-11375

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11375-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11375 targets FH in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen FH fusion protein Ag1934 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fumarate hydratase
Synonyms FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC003108
Gene Symbol FH
Gene ID (NCBI) 2271
RRIDAB_3083900
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07954
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FH antibody CL488-11375Download protocol
Standard Protocols
Click here to view our Standard Protocols