FURIN Monoclonal antibody
FURIN Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D1B9
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, COLO 320 cells, NIH/3T3 cells, JAR cells, HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy FURIN Ready-To-Use IHC Kit. FURIN primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67481-1-Ig targets FURIN in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | Human, Mouse |
| Immunogen | FURIN fusion protein Ag13313 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | furin (paired basic amino acid cleaving enzyme) |
| Synonyms | Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 90-110 kDa |
| GenBank Accession Number | BC012181 |
| Gene Symbol | FURIN |
| Gene ID (NCBI) | 5045 |
| RRID | AB_2882708 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09958 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FURIN antibody 67481-1-Ig | Download protocol |
| IHC protocol for FURIN antibody 67481-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
