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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated FUS/TLS Polyclonal antibody

FUS/TLS Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-11570

Print datasheet

Synonyms

FUS, FUS1, ALS6, ALS, 75 kDa DNA-pairing protein

验证数据展示

  • IF Staining of MCF-7 using CL555-11570

    IF Staining of MCF-7 using CL555-11570

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 FUS/TLS antibody (CL555-11570) at dilution of 1:200.

  • FC experiment of MCF-7 using CL555-11570

    FC experiment of MCF-7 using CL555-11570

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human FUS/TLS (CL555-11570) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

For IHC detection, 60160-1-Ig (mAB) is better.

Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11570-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-11570 targets FUS/TLS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen FUS/TLS fusion protein Ag2150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Synonyms FUS, FUS1, ALS6, ALS, 75 kDa DNA-pairing protein
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC026062
Gene Symbol FUS/TLS
Gene ID (NCBI) 2521
RRIDAB_2919602
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35637
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.

实验方案

Product Specific Protocols
IF protocol for CL555 FUS/TLS antibody CL555-11570Download protocol
FC protocol for CL555 FUS/TLS antibody CL555-11570Download protocol
Standard Protocols
Click here to view our Standard Protocols