• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Factor XII Monoclonal antibody, PBS Only

Factor XII Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2E2F11

Cat No : 66089-1-PBS

Print datasheet

Synonyms

Factor XII, Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

验证数据展示

  • WB analysis of K-562 using 66089-1-Ig (same clone as 66089-1-PBS)

    WB analysis of K-562 using 66089-1-Ig (same clone as 66089-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 66089-1-Ig (same clone as 66089-1-PBS)

    IF Staining of mouse liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

    IF Staining of HepG2 using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66089-1-Ig(Factor XII antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    IHC staining of human liver using 66089-1-Ig (same clone as 66089-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66089-1-PBS in a different storage buffer formulation.

产品信息

66089-1-PBS targets Factor XII in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen Factor XII fusion protein Ag3401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name coagulation factor XII (Hageman factor)
Synonyms Factor XII, Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012390
Gene Symbol F12
Gene ID (NCBI) 2161
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00748
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.