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验证数据展示

  • WB analysis of K-562 using 66089-1-Ig

    WB analysis of K-562 using 66089-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 66089-1-Ig

    IF Staining of mouse liver using 66089-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver using 66089-1-Ig

    IHC staining of human liver using 66089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 66089-1-Ig

    IHC staining of human liver using 66089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

  • IF Staining of HepG2 using 66089-1-Ig

    IF Staining of HepG2 using 66089-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66089-1-Ig

产品说明书

CloneNo. 2E2F11
引用文献(2)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK562 cells, K-562 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66089-1-Ig targets Factor XII in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag3401

Product name:

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor XII (Hageman factor)
别名 F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1
计算分子量 615 aa, 68 kDa
观测分子量 28 kDa
GenBank蛋白编号BC012390
基因名称 Factor XII
Gene ID (NCBI) 2161
RRIDAB_11232031
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
WB protocol for Factor XII antibody 66089-1-IgDownload protocol
IHC protocol for Factor XII antibody 66089-1-IgDownload protocol
IF protocol for Factor XII antibody 66089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Clin Endocrinol Metab

High-Throughput Proteomics and Immunohistochemistry of Orbital Connective Tissue in Graves' Orbitopathy

Authors - Yuanping Hai
View Article
mouseWB

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li
View Article