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CoraLite® Plus 488-conjugated Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E2F11

Cat no : CL488-66089

Print datasheet

Synonyms

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

验证数据展示

  • IF Staining of mouse liver using CL488-66089

    IF Staining of mouse liver using CL488-66089

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Factor XII antibody (CL488-66089, Clone: 2E2F11 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 66089-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66089 targets Factor XII in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen Factor XII fusion protein Ag3401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name coagulation factor XII (Hageman factor)
Synonyms F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012390
Gene Symbol Factor XII
Gene ID (NCBI) 2161
RRIDAB_2883253
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00748
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Factor XII antibody CL488-66089Download protocol
Standard Protocols
Click here to view our Standard Protocols