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验证数据展示

  • IF Staining of HepG2 using CL594-66089

    IF Staining of HepG2 using CL594-66089

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66089 (Factor XII antibody) at dilution of 1:100.

CoraLite®594-conjugated Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF/ICC
Cat No. CL594-66089

产品说明书

CloneNo. 2E2F11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC

别名

F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66089-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66089 targets Factor XII in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag3401

Product name:

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor XII (Hageman factor)
别名 F12, Factor 12, EC:3.4.21.38, Coagulation factor XII, Beta-factor XIIa part 1
计算分子量 615 aa, 68 kDa
观测分子量 28 kDa
GenBank蛋白编号BC012390
基因名称 Factor XII
Gene ID (NCBI) 2161
RRIDAB_2883506
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00748
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
IF protocol for CL594 Factor XII antibody CL594-66089Download protocol
Standard Protocols
Click here to view our Standard Protocols