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G3BP1 Polyclonal antibody
G3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)
Conjugate
Unconjugated
178
验证数据展示
经过测试的应用
Positive WB detected in | C6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13057-2-AP targets G3BP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, RIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken |
Immunogen | G3BP1 fusion protein Ag3728 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
Synonyms | GAP SH3 domain binding protein 1, G3BP-1, G3BP 1, G3BP, EC:3.6.4.12 |
Calculated Molecular Weight | 466 aa, 52 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC006997 |
Gene Symbol | G3BP1 |
Gene ID (NCBI) | 10146 |
RRID | AB_2232034 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13283 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for G3BP1 antibody 13057-2-AP | Download protocol |
IHC protocol for G3BP1 antibody 13057-2-AP | Download protocol |
IF protocol for G3BP1 antibody 13057-2-AP | Download protocol |
IP protocol for G3BP1 antibody 13057-2-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules | ||
Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. | ||
Cell ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. | ||
Cell RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. | ||
Cell Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation. |