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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-13057

Print datasheet

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

验证数据展示

  • IF Staining of HeLa using CL555-13057

    IF Staining of HeLa using CL555-13057

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CoraLite®555 G3BP1 antibody (CL555-13057) at dilution of 1:400.

  • FC experiment of HeLa using CL555-13057

    FC experiment of HeLa using CL555-13057

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human G3BP1 (CL555-13057) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected insodium arsenite treated HeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-13057 targets G3BP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat, mouse
Immunogen G3BP1 fusion protein Ag3728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 1
Synonyms G3BP, G3BP 1, G3BP1, HDH VIII
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC006997
Gene Symbol G3BP1
Gene ID (NCBI) 10146
RRIDAB_2919614
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13283
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

实验方案

Product Specific Protocols
IF protocol for CL555 G3BP1 antibody CL555-13057Download protocol
FC protocol for CL555 G3BP1 antibody CL555-13057Download protocol
Standard Protocols
Click here to view our Standard Protocols