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GABARAPL2-Specific Polyclonal antibody

GABARAPL2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18724-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

ATG8, FLC3A, GABARAPL2, GABARAPL2-Specific, GATE 16, GATE16, GEF 2, GEF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inChloroquine treated HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18724-1-AP targets GABARAPL2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GABA(A) receptor-associated protein-like 2
别名 ATG8, FLC3A, GABARAPL2, GABARAPL2-Specific, GATE 16, GATE16, GEF 2, GEF2
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC005985
基因名称 GABARAPL2
Gene ID (NCBI) 11345
RRIDAB_2232116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

实验方案

Product Specific Protocols
WB protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
IHC protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
IF protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai
  • KO Validated
ratWB

Autophagy

CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.

Authors - Hyun-Kyu An
humanWB

Autophagy

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.

Authors - Jonathan A Stefely
humanIHC

Comput Biol Med

Identifying mitophagy-related genes as prognostic biomarkers and therapeutic targets of gastric carcinoma by integrated analysis of single-cell and bulk-RNA sequencing data

Authors - Zehua Wang
humanWB

Cell Death Dis

Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Authors - Christos E Zois
humanWB, IHC

Neurobiol Dis

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Authors - Tanji Kunikazu K
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