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- KD/KO Validated
CoraLite®594-conjugated GOLGA2/GM130 Polyclonal antibody
GOLGA2/GM130 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy GOLGA2/GM130 Ready-To-Use IHC Kit. GOLGA2/GM130 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11308-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL594-11308 targets GOLGA2/GM130 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | GOLGA2/GM130 fusion protein Ag1848 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Synonyms | GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen |
| Calculated Molecular Weight | 111 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2919763 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein, one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to the Golgi membrane and localized mainly at the cytoplasmic face of the cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in regulating ER-to-Golgi transport and maintaining the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher-order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GOLGA2/GM130 antibody CL594-11308 | Download protocol |
| FC protocol for CL594 GOLGA2/GM130 antibody CL594-11308 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
