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GORASP2 Polyclonal antibody
GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, rat testis tissue, HeLa cells, Raji cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, monkey |
Immunogen | GORASP2 fusion protein Ag0950 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | golgi reassembly stacking protein 2, 55kDa |
Synonyms | GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2 |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC007770 |
Gene Symbol | GORASP2 |
Gene ID (NCBI) | 26003 |
RRID | AB_2113473 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H8Y8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GORASP2 antibody 10598-1-AP | Download protocol |
IHC protocol for GORASP2 antibody 10598-1-AP | Download protocol |
IF protocol for GORASP2 antibody 10598-1-AP | Download protocol |
IP protocol for GORASP2 antibody 10598-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
Dev Cell A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.
| ||
J Clin Invest Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy
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J Cell Biol GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.
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