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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10598

Print datasheet

Synonyms

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

验证数据展示

  • FC experiment of HepG2 using CL594-10598

    FC experiment of HepG2 using CL594-10598

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GORASP2 (CL594-10598) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-10598

    IF Staining of HepG2 using CL594-10598

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GORASP2 antibody (CL594-10598) at dilution of 1:600, CL488-Phalloidin (green).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GORASP2 Ready-To-Use IHC Kit. GORASP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10598-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10598 targets GORASP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen GORASP2 fusion protein Ag0950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi reassembly stacking protein 2, 55kDa
Synonyms GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
RRIDAB_2919740
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8Y8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

实验方案

Product Specific Protocols
IF protocol for CL594 GORASP2 antibody CL594-10598Download protocol
FC protocol for CL594 GORASP2 antibody CL594-10598Download protocol
Standard Protocols
Click here to view our Standard Protocols