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CoraLite® Plus 488-conjugated GORASP2 Monoclonal antibody

GORASP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9A3

Cat no : CL488-66627

Print datasheet

Synonyms

GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6

验证数据展示

  • IF Staining of HepG2 using CL488-66627

    IF Staining of HepG2 using CL488-66627

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GORASP2 antibody (CL488-66627, Clone: 1C9A3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-66627

    FC experiment of HepG2 using CL488-66627

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GORASP2 (CL488-66627, Clone:1C9A3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GORASP2 Ready-To-Use IHC Kit. GORASP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66627-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66627 targets GORASP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat, pig
Immunogen GORASP2 fusion protein Ag17607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name golgi reassembly stacking protein 2, 55kDa
Synonyms GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H8Y8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GORASP2 antibody CL488-66627Download protocol
FC protocol for CL Plus 488 GORASP2 antibody CL488-66627Download protocol
Standard Protocols
Click here to view our Standard Protocols