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验证数据展示

  • WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

    WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

    WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

    WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

    IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

    IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

    IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • Affinity and Kinetic Characterization of 80849-1-RR

    Affinity and Kinetic Characterization of 80849-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

  • Featured Product
  • KD/KO验证

GRP78,BIP Recombinant antibody, PBS Only

GRP78,BIP Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 80849-1-PBS

产品说明书

CloneNo. 5F17

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IP, Indirect ELISA

别名

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

产品信息

80849-1-PBS targets GRP78,BIP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 GRP78,BIP fusion protein Ag2188 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
别名 BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP
计算分子量 78 kDa
观测分子量70,78 kDa
GenBank蛋白编号BC020235
基因名称 GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2918916
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11021
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C