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  • Featured Product
  • KD/KO Validated

GRP78,BIP Recombinant antibody, PBS Only

GRP78,BIP Uni-rAbTM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F17

Cat no : 80849-1-PBS

Print datasheet

Synonyms

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

验证数据展示

  • WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

    WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

    WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

    WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

    IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

    IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

    IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • Affinity and Kinetic Characterization of 80849-1-PBS

    Affinity and Kinetic Characterization of 80849-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

产品信息

80849-1-PBS targets GRP78,BIP in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen GRP78,BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70,78 kDa
GenBank Accession NumberBC020235
Gene Symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2918916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11021
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C