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  • Featured Product
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GRP78,BIP Recombinant antibody

GRP78,BIP Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

5F17

Cat no : 80849-1-RR

Print datasheet

Synonyms

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

验证数据展示

  • WB analysis of HepG2 using 80849-1-RR

    WB analysis of HepG2 using 80849-1-RR

    WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells.

  • WB analysis using 80849-1-RR

    WB analysis using 80849-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 80849-1-RR

    IP experiment of HeLa using 80849-1-RR

    IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug.

  • IHC staining of human colon cancer using 80849-1-RR

    IHC staining of human colon cancer using 80849-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • Affinity and Kinetic Characterization of 80849-1-RR

    Affinity and Kinetic Characterization of 80849-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, RAW264.7 cells, HSC-T6 cells, pig liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

80849-1-RR targets GRP78,BIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivitypig
Immunogen GRP78,BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP
Calculated Molecular Weight 78 kDa
Observed Molecular Weight70,78 kDa
GenBank Accession NumberBC020235
Gene Symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2918916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IHC protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IP protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

J Agric Food Chem

Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota

Authors - Yanan Chen
View Article