CoraLite®594-conjugated GRP94 Monoclonal antibody
GRP94 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4G7C7
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60012-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60012 targets GRP94 in WB, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | WB, IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | GRP94 fusion protein Ag1439 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Synonyms | HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP |
| Calculated Molecular Weight | 96 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC009195 |
| Gene Symbol | GRP94 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2919888 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P14625 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| IF protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| FC protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
