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CoraLite® Plus 488-conjugated GST tag Monoclonal antibody

GST tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B5F8

Cat no : CL488-66001

Print datasheet

Synonyms

GST Tag

验证数据展示

  • WB analysis using CL488-66001

    WB analysis using CL488-66001

    GST tagged recombinant protein lysates were subjected to SDS PAGE followed by western blot with CL488-66001 (GST tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66001 targets GST tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Applications WB Application Description
Tested Reactivity recombinant protein, schistosoma japonicum
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GST tag
Synonyms GST Tag
Calculated Molecular Weight 26 kDa
GenBank Accession NumberFN315687
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 GST tag antibody CL488-66001Download protocol
Standard Protocols
Click here to view our Standard Protocols