• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Galectin-3 Monoclonal antibody

Galectin-3 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C1B2

Cat no : CL594-60207

Print datasheet

Synonyms

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

验证数据展示

  • IF Staining of human thyroid cancer using CL594-60207

    IF Staining of human thyroid cancer using CL594-60207

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

  • IF Staining of human thyroid cancer using CL594-60207

    IF Staining of human thyroid cancer using CL594-60207

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-60207

    FC experiment of HeLa using CL594-60207

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Galectin-3 (CL594-60207, Clone:1C1B2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL594-60207

    IF Staining of HeLa using CL594-60207

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman thyroid cancer tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Galectin-3 Ready-To-Use IHC Kit. Galectin-3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60207-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60207 targets Galectin-3 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Galectin-3 fusion protein Ag6753 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 3
Synonyms Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC001120
Gene Symbol Galectin-3
Gene ID (NCBI) 3958
RRIDAB_2883441
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17931
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

实验方案

Product Specific Protocols
IF protocol for CL594 Galectin-3 antibody CL594-60207Download protocol
FC protocol for CL594 Galectin-3 antibody CL594-60207Download protocol
Standard Protocols
Click here to view our Standard Protocols