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CoraLite® Plus 488-conjugated Giantin Polyclonal antibody

Giantin Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22270

Print datasheet

Synonyms

GCP, GCP372, giantin, GOLGB1, Golgin subfamily B member 1, GOLIM1, Macrogolgin

验证数据展示

  • IF Staining of HepG2 using CL488-22270

    IF Staining of HepG2 using CL488-22270

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Giantin antibody (CL488-22270) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 22270-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22270 targets Giantin in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgin B1, golgi integral membrane protein
Synonyms GCP, GCP372, giantin, GOLGB1, Golgin subfamily B member 1, GOLIM1, Macrogolgin
Calculated Molecular Weight 376 kDa
GenBank Accession NumberNM_004487
Gene Symbol Giantin
Gene ID (NCBI) 2804
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14789
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Giantin is conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Giantin antibody CL488-22270Download protocol
Standard Protocols
Click here to view our Standard Protocols