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HA Tag Recombinant antibody, PBS Only

HA Tag Recombinant Antibody for WB, IP, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6I21

Cat No : 81290-1-PBS

Print datasheet

Synonyms

HA, HA Tag

验证数据展示

  • WB analysis using 81290-1-RR (same clone as 81290-1-PBS)

    WB analysis using 81290-1-RR (same clone as 81290-1-PBS)

    HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293T using 81290-1-RR (same clone as 81290-1-PBS)

    IP experiment of Transfected HEK-293T using 81290-1-RR (same clone as 81290-1-PBS)

    IP result of anti-HA Tag (IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:20000) with Transfected HEK-293T cells lysate 795 ug. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    IF Staining of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

产品信息

81290-1-PBS targets HA Tag in WB, IP, IF, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IP, IF, Indirect ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HA Tag
Synonyms HA, HA Tag
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.