HDAC1 Monoclonal antibody
HDAC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4C4G7
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | human lymphoma tissue, human lung cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:6000-1:24000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66085-1-Ig targets HDAC1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, ChIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | HDAC1 fusion protein Ag19128 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | histone deacetylase 1 |
Synonyms | GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1 |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC000301 |
Gene Symbol | HDAC1 |
Gene ID (NCBI) | 3065 |
RRID | AB_11232033 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13547 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HDAC1 antibody 66085-1-Ig | Download protocol |
IHC protocol for HDAC1 antibody 66085-1-Ig | Download protocol |
IF protocol for HDAC1 antibody 66085-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner. | ||
J Clin Invest The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. | ||
Nat Commun ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin. | ||
Br J Pharmacol Physalin B attenuates liver fibrosis via suppressing LAP2α-HDAC1-mediated deacetylation of the transcription factor GLI1 and hepatic stellate cell activation. | ||
Front Cell Dev Biol LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. | ||
Molecules Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway |