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验证数据展示

  • WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

    WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

    IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC1 antibody included
  • IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

    IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

HDAC1 Monoclonal antibody, PBS Only

HDAC1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66085-1-PBS

产品说明书

CloneNo. 4C4G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HDAC1 Ready-To-Use IHC Kit. HDAC1 primary antibody included.

产品信息

66085-1-PBS targets HDAC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HDAC1 fusion protein Ag19128 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone deacetylase 1
别名 GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1
计算分子量 55 kDa
观测分子量 65 kDa
GenBank蛋白编号BC000301
基因名称 HDAC1
Gene ID (NCBI) 3065
RRIDAB_11232033
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13547
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .