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HDAC1 Monoclonal antibody, PBS Only

HDAC1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C4G7

Cat no : 66085-1-PBS

Print datasheet

Synonyms

GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1

验证数据展示

  • WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

    WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

    IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HDAC1 antibody included
  • IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

    IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HDAC1 Ready-To-Use IHC Kit. HDAC1 primary antibody included.

产品信息

66085-1-PBS targets HDAC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HDAC1 fusion protein Ag19128 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone deacetylase 1
Synonyms GON 10, HD1, HDAC, HDAC1, histone deacetylase 1, RPD3, RPD3L1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC000301
Gene Symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13547
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .