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CoraLite® Plus 488-conjugated HDAC10 Polyclonal antibody

HDAC10 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24913

Print datasheet

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

验证数据展示

  • IF Staining of MCF-7 using CL488-24913

    IF Staining of MCF-7 using CL488-24913

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 HDAC10 antibody (CL488-24913) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC10 Ready-To-Use IHC Kit. HDAC10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24913-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24913 targets HDAC10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen HDAC10 fusion protein Ag18470 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 10
Synonyms Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48
Calculated Molecular Weight 669 aa, 71 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC125083
Gene Symbol HDAC10
Gene ID (NCBI) 83933
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969S8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HDAC10 antibody CL488-24913Download protocol
Standard Protocols
Click here to view our Standard Protocols