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HER2/ErbB2 Monoclonal antibody, PBS Only

HER2/ErbB2 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B12A7

Cat no : 60311-1-PBS

Print datasheet

Synonyms

ERBB2, HER2, HER 2/neu, HER 2, CD340

验证数据展示

  • IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

产品信息

60311-1-PBS targets HER2/ErbB2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen HER2/ErbB2 fusion protein Ag16463 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Synonyms ERBB2, HER2, HER 2/neu, HER 2, CD340
Calculated Molecular Weight 1255 aa, 138 kDa
GenBank Accession NumberBC156755
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
RRIDAB_2881423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04626
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HER2, ErbB2, and Neu is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.