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验证数据展示

  • IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IF Staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • FC experiment of SK-BR-3 using 60311-1-Ig (same clone as 60311-1-PBS)

    FC experiment of SK-BR-3 using 60311-1-Ig (same clone as 60311-1-PBS)

    1x10^6 SK-BR-3 cells were surface stained with 0.2 μg HER2/ErbB2 Monoclonal antibody (60311-1-Ig, Clone: 1B12A7) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG2b isotype control (66360-3-Ig, Clone: 11B8C4) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    IHC staining of human breast cancer using 60311-1-Ig (same clone as 60311-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.

HER2/ErbB2 Monoclonal antibody, PBS Only

HER2/ErbB2 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 60311-1-PBS

产品说明书

CloneNo. 1B12A7

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IHC, IF-P, ELISA

别名

ERBB2, HER2, HER 2/neu, HER 2, CD340

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60311-1-PBS targets HER2/ErbB2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 HER2/ErbB2 fusion protein Ag16463 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
别名 ERBB2, HER2, HER 2/neu, HER 2, CD340
计算分子量 1255 aa, 138 kDa
GenBank蛋白编号BC156755
基因名称 HER2/ErbB2
Gene ID (NCBI) 2064
RRIDAB_2881423
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04626
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HER2, ErbB2, and Neu is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.