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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL647-65218

    FC experiment of human peripheral blood lymphocytes using CL647-65218

    1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Human HLA-DR (CL647-65218, Clone: L243) (blue) or isotype control antibody (black). Cells were not fixed.

CoraLite® Plus 647 Anti-Human HLA-DR (L243)

HLA-DR Monoclonal Antibody for FC
Cat No. CL647-65218

产品说明书

CloneNo. L243

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

Human

应用

FC

别名

HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65218-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65218 targets HLA-DR in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 major histocompatibility complex, class II, DR alpha
别名 HLA DRA, HLA DRB, HLA-DR, HLA-DRA, HLA-DRB, MHC class II antigen DR
计算分子量 254 aa, 29 kDa
GenBank蛋白编号BC032350
基因名称 HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
RRIDAB_2883729
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01903
储存缓冲液 PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HLA-DR antibody CL647-65218Download protocol
Standard Protocols
Click here to view our Standard Protocols