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  • Featured Product
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HSP90 Polyclonal antibody

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 13171-1-AP

Print datasheet

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

验证数据展示

  • WB analysis using 13171-1-AP

    WB analysis using 13171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13171-1-AP (HSP90 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13171-1-AP

    IP experiment of K-562 using 13171-1-AP

    IP result of anti-HSP90 (IP:13171-1-AP, 3ug; Detection:13171-1-AP 1:500) with K-562 cells lysate 2000ug.

  • IHC staining of mouse testis using 13171-1-AP

    IHC staining of mouse testis using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AA1 antibody included
  • IHC staining of mouse testis using 13171-1-AP

    IHC staining of mouse testis using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13171-1-AP (HSP90 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AA1 antibody included
  • IHC staining of human colon using 13171-1-AP

    IHC staining of human colon using 13171-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13171-1-AP (HSP90 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AA1 antibody included
  • IF Staining of HepG2 using 13171-1-AP

    IF Staining of HepG2 using 13171-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 13171-1-AP

    IF Staining of HepG2 using 13171-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (13171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HeLa using 13171-1-AP

    FC experiment of HeLa using 13171-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HSP90 Polyclonal antibody (13171-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inmouse testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AA1 Ready-To-Use IHC Kit. HSP90AA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1600-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13171-1-AP targets HSP90 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, chicken, bovine, sheep, fish, carp
Immunogen HSP90 fusion protein Ag3826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Synonyms HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1
Calculated Molecular Weight 853 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90
Gene ID (NCBI) 3320
RRIDAB_2120924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

实验方案

Product Specific Protocols
WB protocol for HSP90 antibody 13171-1-APDownload protocol
IHC protocol for HSP90 antibody 13171-1-APDownload protocol
IF protocol for HSP90 antibody 13171-1-APDownload protocol
IP protocol for HSP90 antibody 13171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

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CoIP

Nat Cell Biol

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mouse,ratWB

Nat Commun

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