验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13171 targets HSP90 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | HSP90 fusion protein Ag3826 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Synonyms | HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1 |
| Calculated Molecular Weight | 853 aa, 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC023006 |
| Gene Symbol | HSP90 |
| Gene ID (NCBI) | 3320 |
| RRID | AB_3672559 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07900 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HSP90 antibody CL488-13171 | Download protocol |
| FC protocol for CL Plus 488 HSP90 antibody CL488-13171 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |