HSP90 Monoclonal antibody
HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
3F11C1
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60318-1-Ig targets HSP90 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, RIP |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken |
Immunogen | HSP90 fusion protein Ag3826 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
Synonyms | Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2 |
Calculated Molecular Weight | 853 aa, 90 kDa |
Observed Molecular Weight | 85-90 kDa |
GenBank Accession Number | BC023006 |
Gene Symbol | HSP90 |
Gene ID (NCBI) | 3320 |
RRID | AB_2881429 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P07900 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HSP90 antibody 60318-1-Ig | Download protocol |
IHC protocol for HSP90 antibody 60318-1-Ig | Download protocol |
IF protocol for HSP90 antibody 60318-1-Ig | Download protocol |
IP protocol for HSP90 antibody 60318-1-Ig | Download protocol |
FC protocol for HSP90 antibody 60318-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Aging Cell Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis. | ||
Mol Cancer Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation. | ||
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Cell Death Dis HOXC11 drives lung adenocarcinoma progression through transcriptional regulation of SPHK1 |