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CoraLite® Plus 647-conjugated HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F11C1

Cat no : CL647-60318

Print datasheet

Synonyms

FLJ31884, Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

验证数据展示

  • FC experiment of K-562 using CL647-60318

    FC experiment of K-562 using CL647-60318

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human HSP90 (CL647-60318, Clone:3F11C1) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AA1 Ready-To-Use IHC Kit. HSP90AA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60318-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60318 targets HSP90 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen HSP90 fusion protein Ag3826 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Synonyms FLJ31884, Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2
Calculated Molecular Weight 853 aa, 90 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90
Gene ID (NCBI) 3320
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07900
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HSP90 antibody CL647-60318Download protocol
Standard Protocols
Click here to view our Standard Protocols