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  • Featured Product
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HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 11405-1-AP

Print datasheet

Synonyms

HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta

验证数据展示

  • WB analysis using 11405-1-AP

    WB analysis using 11405-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11405-1-AP

    WB analysis using 11405-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11405-1-AP

    WB analysis of HEK-293 using 11405-1-AP

    WB result of HSP90AB1 antibody (11405-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

  • IHC staining of human pancreas cancer using 11405-1-AP

    IHC staining of human pancreas cancer using 11405-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IHC staining of human pancreas cancer using 11405-1-AP

    IHC staining of human pancreas cancer using 11405-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IP experiment of NIH/3T3 using 11405-1-AP

    IP experiment of NIH/3T3 using 11405-1-AP

    IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

  • IHC staining of human colon using 11405-1-AP

    IHC staining of human colon using 11405-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP90AB1 antibody included
  • IF Staining of HepG2 using 11405-1-AP

    IF Staining of HepG2 using 11405-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90AB1 antibody (11405-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 11405-1-AP

    FC experiment of HeLa using 11405-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP90AB1 Polyclonal antibody (11405-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11405-1-AP targets HSP90AB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, sheep, deer
Immunogen HSP90AB1 fusion protein Ag1947 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Synonyms HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta
Calculated Molecular Weight 723 aa, 83 kDa
Observed Molecular Weight 83-90 kDa
GenBank Accession NumberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2121207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

实验方案

Product Specific Protocols
WB protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IHC protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IF protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IP protocol for HSP90AB1 antibody 11405-1-APDownload protocol
FC protocol for HSP90AB1 antibody 11405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
View Article
pigWB

Autophagy

Induction of autophagy and suppression of type I IFN secretion by CSFV.

Authors - Baoming Xie
View Article
WB

Cell Rep

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

Authors - Carissa L Sirois
View Article
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
View Article
humanWB

Anal Chem

Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.

Authors - Xiaolei Zhang
View Article
sheepIF

PLoS Pathog

A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.

Authors - Thomas Labadie
View Article