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KD/KO Validated

HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Publications

Cat no : 11405-1-AP

Print datasheet

Synonyms

HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta

验证数据展示

WB analysis using 11405-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 11405-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11405-1-AP
WB result of HSP90AB1 antibody (11405-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.
IHC staining of human pancreas cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP90AB1 antibody included
IHC staining of human pancreas cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP90AB1 antibody included
IP experiment of NIH/3T3 using 11405-1-AP
IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.
IHC staining of human colon using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP90AB1 antibody included
IF Staining of HepG2 using 11405-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90AB1 antibody (11405-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11405-1-AP

Size

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经过测试的应用

Positive WB detected in	PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 29 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 3 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

产品信息

11405-1-AP targets HSP90AB1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, sheep, deer
Immunogen	HSP90AB1 fusion protein Ag1947 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	heat shock protein 90kDa alpha (cytosolic), class B member 1
Synonyms	HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta
Calculated Molecular Weight	723 aa, 83 kDa
Observed Molecular Weight	83-90 kDa
GenBank Accession Number	BC014485
Gene Symbol	HSP90AB1
Gene ID (NCBI)	3326
RRID	AB_2121207
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08238
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

实验方案

Product Specific Protocols
WB protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IHC protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IF protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IP protocol for HSP90AB1 antibody 11405-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IP	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
pig	WB	Autophagy Induction of autophagy and suppression of type I IFN secretion by CSFV. Authors - Baoming Xie View Article
	WB	Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons Authors - Carissa L Sirois View Article
human	WB	Haematologica Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Authors - Stephan Michalik View Article
human	WB	Anal Chem Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale. Authors - Xiaolei Zhang View Article
sheep	IF	PLoS Pathog A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. Authors - Thomas Labadie View Article

HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 11405-1-AP

WB analysis using 11405-1-AP

WB analysis of HEK-293 using 11405-1-AP

IHC staining of human pancreas cancer using 11405-1-AP

IHC staining of human pancreas cancer using 11405-1-AP

IP experiment of NIH/3T3 using 11405-1-AP

IHC staining of human colon using 11405-1-AP

IF Staining of HepG2 using 11405-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Induction of autophagy and suppression of type I IFN secretion by CSFV.

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.

A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.